primary human coronary artery vascular endothelial cells  (Lonza)

Journal: Contrast Media & Molecular Imaging

Article Title: Regulation of the Proliferation of Diabetic Vascular Endothelial Cells by Degrading Endothelial Cell Functional Genes with QKI-7

doi: 10.1155/2022/6177809

Figure Lengend Snippet: QKI-7 was highly expressed in patients with diabetes and atherosclerosis. ∗ p < 0.05, taking the normal patient group as the control. From clinical blood samples of normal patients, diabetic patients, and diabetic patients with atherosclerosis, the expression level of QKI-7 was detected by RT-qPCR (a) and the ELISA (b) assay to detect the secretory expression level of endothelial cell dysfunction markers E-selection, ICAM-1, and VCAM-1 in blood samples.

Article Snippet: Human vascular endothelial cells were purchased from ATCC, No.: PCS-100-020.

Techniques: Control, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Selection

Journal: Contrast Media & Molecular Imaging

Article Title: Regulation of the Proliferation of Diabetic Vascular Endothelial Cells by Degrading Endothelial Cell Functional Genes with QKI-7

doi: 10.1155/2022/6177809

Figure Lengend Snippet: QKI-7 with high-glucose treatment inhibited the expression of CD144, NLGN1, and TSG6 in endothelial cells and promoted cell proliferation. ∗ p < 0.05, with the untreated group as the control. Treated endothelial cells induced by differentiation of human pluripotent stem cells with 50 mM high concentration glucose, and detection of the expression levels of CD144, NLGN1, and TSG6 by RT-qPCR (a) and western blot (b). CCK-8 was used to evaluate the changes in cell proliferation efficiency of the high-glycemic treatment group and the untreated group (c).

Article Snippet: Human vascular endothelial cells were purchased from ATCC, No.: PCS-100-020.

Techniques: Expressing, Control, Concentration Assay, Quantitative RT-PCR, Western Blot, CCK-8 Assay

Journal: Contrast Media & Molecular Imaging

Article Title: Regulation of the Proliferation of Diabetic Vascular Endothelial Cells by Degrading Endothelial Cell Functional Genes with QKI-7

doi: 10.1155/2022/6177809

Figure Lengend Snippet: The effect of QKI-7 overexpression on endothelial cell functional genes and proliferation efficiency QKI-7 was overexpressed in endothelial cells induced by human pluripotent stem cells (a), which significantly reduced the transcription level (b) and translation level (c) of CD144, NLGN1, and TSG6 and promoted the proliferation of endothelial cells (d). ∗ p < 0.05, with the untreated group as the control.

Article Snippet: Human vascular endothelial cells were purchased from ATCC, No.: PCS-100-020.

Techniques: Over Expression, Functional Assay, Control

Journal: Contrast Media & Molecular Imaging

Article Title: Regulation of the Proliferation of Diabetic Vascular Endothelial Cells by Degrading Endothelial Cell Functional Genes with QKI-7

doi: 10.1155/2022/6177809

Figure Lengend Snippet: QKI-7 overexpression increased the expression levels of inflammatory factors of TNF- α , IL-1 β , and IFN- γ . ∗ p < 0.05, with the untreated group as the control. The overexpression of QKI-7 in endothelial cells induced by human pluripotent stem cells significantly increased the transcription level (a) and translation level (b) of the cytokines TNF- α , IL-1 β , and IFN- γ . ELISA results showed that the extracellular secretion level of PGI2 rose noticeably (c).

Article Snippet: Human vascular endothelial cells were purchased from ATCC, No.: PCS-100-020.

Techniques: Over Expression, Expressing, Control, Enzyme-linked Immunosorbent Assay

Journal: Contrast Media & Molecular Imaging

Article Title: Regulation of the Proliferation of Diabetic Vascular Endothelial Cells by Degrading Endothelial Cell Functional Genes with QKI-7

doi: 10.1155/2022/6177809

Figure Lengend Snippet: QKI-7 knockdown significantly increased the expression of CD144, NLGN1, and TSG6 and reduced cell proliferation efficiency. ∗ p < 0.05, with the nonknockdown group as control. RT-qPCR (a, b) and western blot (c) detected the transcription and translation levels of key endothelial cell genes QKI-7, CD144, NLGN1, and TSG6; the CCK-8 experiment detected the change in cell proliferation efficiency (d).

Article Snippet: Human vascular endothelial cells were purchased from ATCC, No.: PCS-100-020.

Techniques: Knockdown, Expressing, Control, Quantitative RT-PCR, Western Blot, CCK-8 Assay